Product Description
GeneMATRIX Cell Culture DNA Purification Kit is designed for rapid purification of total DNA (genomic, mitochondrial) from animal or human cell culture. Purified DNA is free of contaminants, such as: RNA, proteins, lipids, dyes, detergents, organic inhibitors of enzymatic reactions, buffers, salts, divalent cations, among others.
Cells sample is lysed in the presence of special buffer containing large amounts of chaotropic ions and Proteinase K. Proteinase K digests cellular proteins, including stripping-off DNA of all bound proteins, among them nucleases. Appropriate conditions for binding of DNA to the GeneMATRIX resin is created by addition of ethanol to the lysate. During brief centrifugation step DNA binds to the silica membrane in the spin-column, while contaminants pass through. Traces of contaminants remaining on the resin are efficiently removed in two wash steps. High-quality cellular DNA is then eluted in low salt buffer, e.g.: Tris-HCl, TE or water. Isolated DNA is ready for downstream applications without the need for ethanol precipitation.
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