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Steril – Bioban

BIOBAN cabinets produced by Steril are designed and manufactured to fulfil and satisfy the European Norm EN12469.
Routinelly used in laboratory of microbiology, virology, haematology and cell culture.
Furthermore, used in innovative fields of scientific research and above all in the manipulation of:
• Recombinant DNA
• Oncogenic Virus
• Pathogenic agents
For a correct use and application of the cabinet here described, it is necessary to refer to the appropriate standard norm and rules in force in the country where the cabinet is used.
Technical Specification
Back panel in stainless steel AISI 304L “2B” finishing, thickness 12/10.
Working surface in stainless steel AISI 304L “2B” finishing, divided in sectors removable and autoclavable (4 sectors for mod. 48 and 6 sectors for mod. 72), available both in perforated and solid version.
Sidewalls in toughened safety glass.
Front window made in toughened safety glass electrically driven, 2,5° slope for optimum ergonomic worker posture.
Lighting on working surface by means of fluorescent lighting fitted in non-contaminated area.
Minimum level of lighting 1.000 lux.
Construction structure in steel epoxy-polyester powder coated, resistant to the most common industrial disinfectants.
Ventilation system by means of n°1 motor blower dedicated to the one-way airflow in the working chamber, equal to 70% of the total involved air and to exhaust the remaining 30%.
The motor blower is centrifugal type, direct driven motor with double aspiration and protection factor IP55.
Air filtration, both recirculate and exhaust air by means of H14 HEPA filters with efficiency 99,995% MPPS as per EN 1822 (EU14 with efficiency 99,999% tested with DOP/DOS @ 0.3 ?m). Both filters will be tested with scanning method for integrity with DOP/DOS test.
UV germicidal lamp on back stainless steel panel.

Command and Controls
Air ventilation control by means of automatic regulation of the revolution velocity of the motor blower. One high-resolution flow rate volumetric device, directly interfaced to microprocessor, achieves the automatic regulation. The microprocessor guarantees the activity of the motor blower and controls the optimal function even in presence of effects caused by progressive clogging of the HEPA filters.
Alarm device optical (red signal light visible from working position and display message) and acoustic (buzzer) type, activated, at real time, by the microprocessor, showing the kind of alarm on a wide alpha numeric display.

Monitoring with alarms of the following parameters:
• Exhaust flow rate out of the pre-set range;
• Laminar air flow rate out of the pre-set range;
• Failure of the motor blower;
• Front window in wrong position;

Alpha numeric display showing:
• Laminar air flow speed in m/s;
• Date and time
• Hours counters for cabinet life
• UV Lamp run hours
• HEPA filters run hours
• Date of last HEPA filters change
• Date of last service
• Date of last power failure
• Change language
• Change password
• Setting UV timer program
• Setting MSC decontamination program

Standard features
• Fluorescent lighting
• Power socket 4A, IP 55 (internal back panel-right side).
• DOP/DOS 100% test inlet hose-barb port (internal under worktop – left side)
• 3 holes (19 mm diameter) on both side windows (for fluid taps installation or other connections) with plastic caps.
• UV germicidal lamp 30 W on back stainless steel panel wavelength 253.7 nm (UV-C).

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Steril – CTH

Performances
Operator Protection
The containment index, evaluated on the total surface of the front working opening, is equal or less than 5 CFU for not disturbance test or Apf equal or more than 1 x 105 according EN12469.
Product Protection
Sterility in the working area higher than ISO Class 5 according to ISO EN 14644 (Class 100/M3.5 according to Federal Standard 209E) @ 0.3 and 0.5 μm particles. Equal or less than 5 CFU per test, according product protection test EN12469 and Equal or less than 2 CFU per test, according cross contamination test EN12469.
Environmental Protection
By filtration of the exhaust air through H14 HEPA filter with efficiency 99,995% MPPS as per EN 1822 (EU14 with efficiency 99,999% tested with DOP/DOS @ 0.3 μm). It is suggested, except for particular cases, to duct outside the exhaust air as recommended by DIN 12980.
Command and Controls
Air ventilation control by means of automatic regulation of the revolution velocity of the motor blower. Two highresolution flow rate volumetric devices, directly interfaced to microprocessor, achieve the automatic regulation. The microprocessor guarantees the activity of the motor blowers and controls the optimal function even in presence of effects caused by progressive clogging of the HEPA filters. Alarm device optical (red signal light visible from working position and display message) and acoustic (buzzer) type, activated, at real time, by the microprocessor, with indication of type of alarm activate showing on a wide alpha numeric display.
Monitoring with alarms of the following parameters:
Exhaust flow rate out of the pre-set range;
Laminar air flow velocity out of the pre-set range;
Front window in wrong position;
Alpha numeric display showing:
Laminar air flow speed in m/s;
Exhaust air flow rate in m3/h
Date and time
Hours counters for cabinet life
UV Lamp run hours
HEPA filters run hours
Date of last HEPA filters change
Date of last service
Date of last power failure
Change language
Change password
Setting UV timer program
Setting MSC decontamination program

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Steril – GEMINI

Applications
Normally used in laboratory of microbiology, molecular biology and, furthermore, used in innovative fields of scientific research and above all in the manipulation of:
Quality Control in pharmaceutical and food industries
Micro mechanical assembly
DNA/RNA amplification and thermo-cycling
Technical Specification
Back panel and working tray in stainless steel AISI 304L “2B” finishing, perforated.
Front window and side walls in toughened glass.
Lighting on working surface by means of 1 fluorescent lamps of 15W
Construction structure in steel epoxy-polyester powder coated, resistant to the most common industrial disinfectants.
Ventilation system by means of n°1 motor blower dedicated to the one-way airflow in the working chamber, equal to 95% of the total involved air and to exhaust the remaining 5%.
The motor blower is centrifugal type, direct driven motor with double aspiration and protection factor IP22.
Air filtration by means of HEPA “H14 type” filter (refer to DIN 24184 and BS 3928) with efficiency of 99,999% at 0,3 micron particles size with DOP/DOS test (as defined to EN1822).
Command and Controls
Air ventilation control by means of manual electronic regulation of the revolution velocity of the motorblower.

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Steril – HELIOS

Helios are horizontal laminar airflow cabinets produced by Steril and designed for the
situation where is requested the product protection from dangerous effects due
to uncontrolled diffusion of air-transported contaminants from the
environmental to the product during its handling.
Applications
Normally used in laboratory of microbiology and cell culture.
Furthermore, used in innovative fields of scientific research and above all in the manipulation of:
Quality Control in food industries
Micro mechanical assembly
Preparation of nutritional parentheral bags
IVF
Performances
Product Protection
Sterility in the working area higher than ISO 5 class according to ISO EN 14644-1 @ 0,3 and 0,5 µm particles size (Class 100/M3.5 according to Federal Standard 209E) with a laminar air flow velocity of 0,40 m/s +/-20%.
Since HELIOS is not a biosafety cabinet, it is not suitable for handling pathogens components.
Technical Specification
Working surface in stainless steel AISI 304L “2B” finishing with 15 mm lift (see picture).
Side walls in toughened safety glass with 3 holes 19 mm diameter for the easy passage of external connection of fluids and/or cables without necessity of factory assembly.
Lighting on working surface by means of 2 fluorescent lamps of 36W fitted in non-contaminated area for HELIOS 48 and 58W for HELIOS 72.
Construction structure in steel epoxy-polyester powder coated, resistant to the most common industrial disinfectants.
Ventilation system by means of n°1 motor blower for HELIOS 48 dedicated to the one-way airflow in the working chamber.
HELIOS 72 is supplied with n° 2 motor blowers in parallel way system
The motor blower is centrifugal type, direct driven motor with double aspiration and protection factor IP55.
Air filtration by means of disposable high retention capacity pre-filters and H14 HEPA filters with efficiency 99,995% MPPS according to EN 1822 (99,999% tested with DOP @ 0,3 µm).
Protection grid, for HEPA filter made by anodized aluminium.
Standard features
Automatic air velocity regulation
Fluorescent lights
Power socket 4 A UNEL IP 55
DOP/DOS 100% test inlet hose-barb port

Optional features
Steel support stand epoxy polyester coated
Chest of 3 drawers on pivoting wheels in steel epoxy coated
30W germicidal lamp with front screen, wavelength 253.7 nm (UV-C)
Gas manual tap manufactured in compliance with UNI/CIG regulations. Tap is provided with the automatic safety valve for interruption in case of electric blackout and/or air velocity out of pre-set range. Terminal connection for 6 mm diameter gas line.
Bunsen burner with automatic switch ignition
Bunsen burner with automatic foot switch ignition
Compressed air line with manual tap
Nitrogen line with manual tap
Vacuum line with manual tap

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Steril – Powder Safe

This POWDER SAFE cabinet is a vertical laminar flow in ISO 5 Class according to ISO EN 14644-1 @ 0,3 and 0,5 m particles size (Class 100/M3.5 according to Federal Standard 209E). The cabinet is designed with part of air recirculated and part of air exhausted outside the equipment through H14 HEPA filters (according to EN 1822).
The operating cycle described above together with an inflow air barrier assure a slight depression and, therefore, isolation from the surrounding area.
Application
The identified and carefully estimated factors considered as essential, with particular reference to process and safety requirements, are:
• protection of the product;
• protection and safety of the operator;
• containment of handled product;
• cleanness of working area;
• ergonomic factors.
The operator and the environmental protection and product safety from cross contamination are obtained by appropriate flows permanently monitored by a microprocessor automatic control system.
The operator and the environmental protection are achieved exhausting 40% of air through an HEPA filter. This maintains the cabinet in negative pressure and creates an air barrier of more than 0,40 m/s. The recirculation of the remaining 60% of the air on the working area in laminar air flow condition at a mean 0,30 m/s through another HEPA filter guarantees the cleanness of the working area, avoiding cross contamination and any perturbation or dispersion of dust.
Moreover the specific construction of the cabinet, special fixing devices on the fan together with a marble anti-vibration support (optional) allow the use of balance for measures up to 0.01 mg.

Standard features
Fluorescent lights.
N. 2 Power sockets 4 A GERMAN TYPE IP 55 (internal back panel right and left side)
DOP/DOS 100% test inlet hose-barb port (internal under worktop – left side).
3 holes (19 mm diameter) on both side windows (for fluid taps installation or Balances/PC connections) with plastic caps.
Optional features
Epoxy polyester coated steel support stand
Chest of 3 drawers on pivoting wheels in steel epoxy coated
Additional electrical power socket (right or left internal back panel)
Anti-vibration marble stone support tested for measures up to 0,01 mg
Compressed air line with manual tap
Nitrogen line with manual tap
Vacuum air line with manual tap
Gas line with manual tap (internal back panel – right side) manufactured in compliance with UNI/CIG regulations, piping made of copper and hose-bard 10 mm. diameter (external) for the connection to the main line. The gas line is provided with electrical automatic safety valve for interruption in case of electric blackout and/or ventilation failure.

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Steril – SterilSafe

STERILSAFE® series are vertical laminar flow biohazard cabinets, Class II A1-A2, certified according to the European Standards EN12469–2000.
Dual motors system with auto adjustment of airflow within the working space according to filter loading conditions.
Built-in V worktop. Anti-blocking air return slots.
Electrical and angled front window.
Right side glass with three port holes to allow the insertion of facilities inside tha cabinet.
Powerful lightning.
Integrated UV Lamp.
Optimal angle, of the front panel, to keep a clear view and increase the comfort.
The wide touch screen, the tilting electrical front glass, quiet, comfortable, the easy to clean and to use combined with top safety make STERILSAFE ® one of the top vertical laminar flow biohazard cabinets in the market. Designed to match all GMP guidelines STERILSAFE ® series key features includes germicidal UV lamp installed on a tilt-away holder, very bright working area, supporting structure epoxy coated, ventilation system by means of two centrifugal fans with a degree of protection IP 55, recirculation and exhaust air filtration by HEPA H14 filters according to EN1822 (with 99.995% efficiency MPPS).
STERILSAFE ® will make their daily work fatigue-free and an enjoyable experience.

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Steril – TOPFLOW 2 Mobile LAF cabinet for laboratory animal cages emptying

Top Flow 2 is a single fan cabinet manufactured like a Class I microbiological safety cabinet with inward air velocity more than 0.80 m/s, 100% exhausted through an HEPA filter and visual/acoustic alarms in compliance to European standard EN 12469: 2000. Top Flow 2 is designed for emptying out and cleaning of cages, in a sterile area, with protection given to operator and environment. The unit is supplied with one garbage trolley with stainless steel wheels.
Technical characteristics
Exhaust HEPA filter: efficiency 99.95% MPPS H13 (99.99% 0.3 m DOP test), 305x610x292; with protection grid in order to avoid unattended blockage by foreign objects. Pre filters: EU3 cells according to EN 779 400x500x22mm with quick fixing system and access through an hinged panel from the work area. Prefilter protection grid made of stainless steel AISI 304L scotch brite finish. Electronic board with microprocessor system to control all functioning of the cabinet: and automatic regulation of the fan speed fan to compensate the blockage of the filter, alarms, manages of the utilities.
Monitoring with alarms of the following parameters:
Intake air flow velocity out of the pre-set range;
Failure of the motor blower;
Front window in wrong position;

Alphanumeric display showing:
Laminar air flow speed in m/s;
Date and time
Hours counters for cabinet life
UV Lamp run hours
HEPA filters run hours
Date of last HEPA filters change
Date of last service
Date of last power failure
Change language
Change password
Soft touch control panel.
Intake airflow velocity more than 0.8m/s* (as per Class I bio-safety cabinet) automatically adjusted and monitored by the microprocessor system. (*Corresponding to 800 m3/h).
External structure made of steel epoxy polyester powder coated, resistant to the most common industrial disinfectant.
Hinged solid work surface made of stainless steel AISI 304L scotch brite finish supported by two gas springs, to allow access to the garbage bag to operator in standing position while the cabinet is running, with transition adapter to waste material into the garbage bag.
Hinged window in safety laminated glass framed with anodised aluminium profile supported by two gas springs.
Safety glass side windows.
Double inlet centrifugal fan with direct driven external rotor motor to improve efficiency end reduce noise (less than 58 dB).
Stainless steel swivelling wheels with brake.
Easy access to all the dirty zones inside the cabinet.
30-Watt fluorescent lamp in non-contaminated area.
Garbage bag trolley made of stainless steel AISI 304L scotch brite finish and plastic with swivelling wheels.

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Steril – TOPFLOW 3 Mobile LAF cabinet for LAB animals cages changing

Top Flow 3 is a double fan, double side operated vertical laminar airflow cabinet, with partial recirculation. This unit is manufactured like a Class II microbiological safety cabinet with 0.40 m/s mean LAF velocity, front opening inward air velocity more than 0.40 m/s and visual/acoustic alarms in compliance to European standard EN 12469: 2000.
Top Flow 3 is specifically designed for manipulating and passing of mice or other experimental animals, from dirty cages to clean cages, in a sterile area, with protection for operator, animal and environment. An electrical driven lifting system of the whole cabinet allows the optimum ergonomic worker posture.
Main and Exhaust HEPA filter: H14, 99.995% efficiency MPPS according to EN 1822 (99,999% DOP test efficiency at 0,3 ?m);
Main and Exhaust filters HEPA are fitted according to negative seal principle to avoid any escape of contaminated particles and they are testable with DOP for filter integrity).
Pre-filter (F5) installed below the working surface, to extend filters life and to prevent blockage of motor blower
Cleanness of air in working area is class ISO 5 @ 0.3 and 0.3 ?m particles according to ISO EN 14644-1 (Class M3.5/100 according to FS209E).
Exterior structure in carbon-steel (12/10) white coated with epoxy polyester powder RAL 9010
Work surface and harm-rest in stainless steel AISI 304L scotch brite
Drain basin under the aspiration part of the work surface, made in stainless steel AISI 304L with rounded edge;
Protection grid, for main HEPA filter made by anodized aluminium.
Side windows in 5 mm Safety Glass.
N° 2 hinged frontal screens, one per side, in 4+4 mm double layer Laminated Safety Glass with anodised aluminium frame with aperture height in working position of 290 mm. Opening angle of 150° to obtain a free aperture section of 1160 x 530 mm (w x h).
N° 2 soft touch control panel, one in each side in order to work with a cabinet like two independent cabinets (see description below).
N° 2 front closure in plexiglas.
Fluorescent lights installed outside the contamination area (2x15Watt) to obtain 400 lux (unbothered for animals).
Easy maintenance of the motorblower and of the HEPA filters from both front side of cabinet.
Two lateral handles for movement and positioning.
External dimension: 1400 x 840 x 1980 mm (wxdxh).
Working chamber dimension: 1160 x 700 x 674 mm (wxdxh).
Base supplied with stainless steel wheels and brakes (125 mm diameter) and electrical adjustable device for regulation of work surface height from 800 mm to 1100 mm; Level of work surface is 100 mm under level of harm-rest.

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Steril – VBH Compact

VBH Compact cabinets produced by Steril are designed to fulfill and satisfy the European Norm EN12469 and country standards (presently superseded by EN12469) like German DIN 12950/10, French NF44-201 and English BS 5726.
VBH Compact cabinets are manufactured with a frontal working opening for the aspiration of 30% of the total air involved, at a minimum velocity of 0,40 m/s, and the remaining 70% is recirculated through a HEPA filter in the working chamber with a mean laminar flow velocity of 0,40 m/s (as defined by European standard EN 12469: 2000). The containment index, evaluated on the total surface of the front working opening, corresponds to APF (Aperture Protection Factor) equal or more than 1 x 105 according EN12469. The microprocessor monitors in real time all critical parameters of the unit (laminar air flow velocity, exhaust flow rate and position of the front screen) and controls the optimal function of the fans even if in presence of effects caused by progressive clogging of the HEPA filters.
TECHNICAL SPECIFICATION
Work chamber and spillage tray in stainless steel AISI 304L “2B” finishing. Working surface in stainless steel AISI 304L “2B” finishing, removable and autoclavable in perforated and solid version. Hinged front window, in laminated safety glass; ergonomic opening 150°. Maximum free height with open screen: 540 mm. Full vision height of 535 mm at operational condition. Ergonomic front closure panel in anodized aluminium complete of germicidal UV lamp. Lighting on working surface by means of 2 fluorescent electronic lamps fitted in non-contaminated area. Construction structure in steel epoxy-polyester powder painted. Ventilation system by means of n°2 IP55 protection factor motor blowers. Recirculating and exhaust air filtration achieved by H14 HEPA filters as per EN 1822 (with efficiency 99.995% MPPS). Maintenance completely from the front of the cabinet.
SAFETY DEVICES
Microprocessor monitoring with alarms of the following parameters:
Exhaust flow rate out of the pre-set range;
Laminar air flow velocity out of the pre-set range;
Front window in wrong position;
Fans failure.
STANDARD ACCESSORIES
Decontamination program.
UV Germicidal lamp.
UV timer program.
Power socket 4A IP55.
Vacuum air line.
Fuel gas line.
OPTIONAL ACCESSORIES
PC interface for monitoring and remote servicing with lithium battery.
Double HEPA exhaust filter.
Activated carbon exhaust filter.
Support stand.
Chest of 3 drawers on pivoting wheels.

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Cabinets

Steril – Bioban

Steril – CTH

Steril – GEMINI

Steril – HELIOS

Steril – Powder Safe

Steril – SterilSafe

Steril – TOPFLOW 2 Mobile LAF cabinet for laboratory animal cages emptying

Steril – TOPFLOW 3 Mobile LAF cabinet for LAB animals cages changing

Steril – VBH Compact

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